Monday, July 27, 2009
Of Weekend Work and Fortune Tellers...
Sunday, July 26, 2009
This is true procrastination...
Experiment design.
It's a relatively simple antibody workup. But not so simple when there are 4 or 5 antibodies that needs to be done on relatively few samples. We're only harvesting 2 mice spleens and 4 mesenteric lymph nodes. One mouse spleen might yield 10^8 lymphocytes, if my technique can be called "up to scratch". That's not a lot of cells to stain. Another practical difficulty is the selection of fluorochromes for Flow Cytometry. Wouldn't it have been nice to have all the colours in the world? But at most there're only 5 available for our use. Not to mention the cells still need to be stained with the usual anti-CD4 and anti-Valpha/beta to select CD4+ T lymphocytes.
Experiment starts 8:00am tomorrow morning.
I'm sitting here feeling anything but productive. Must be the whether, I think.
Ah well, at least I'll get to have fun searching for little white blobs of Lymph node in the big white fatty mass of the mice mesentery tomorrow. That's always something to look forward to.
Procrastination... Not...
However, this week I've been on a steep learning curve, with the first and second day running around in the lab preparing artificial CSF and RNA/DNA mediums, trying to remember which chemicals have to be kept in under 4 degrees, -20 degrees and -80 degrees, or in the water bath, and when to switch the temperature, and when to remove them from those temperatures. Huge pain in the arse, I might say, that the supervisor seems to place a large amount of trust in my memory by giving me instructions as a passing comment. One time, I forgot to remove the artificial CSF from the magnetic stirrer on my second day and left the solution at room temperature overnight, and so I had to remake a new batch on the day next and so there was a delay in the rat brain harvest surgery.
There were 2 cool events that happened in my first week, one of them by chance. On Tuesday my lab partner Gabi was showing me on how to weigh rats when one of the status epilepticus rats went into grade 5, then 4, then 6 seizure. Grade 6 is most terrifying and is also known as 'popcorn' among the lab people - because it is when the rat jerks violently up and down like popcorn popping. I thought 'popcorn' was a bit of an understatement because the rat jumped up and down so violently that the lid above broke off and the creature unconciously made its escape. Thankfully, Gabi was quick enough to lift it and hoist it back into the cage before the rat fell off the table. The second cool event (but perhaps not too cool) was the rat brain harvest. Rats had to be guilotined for the brain harvest and since general anaesthetics will alter the methylation status in the brain, decapitation had to be done with the rat fully awake (and writhing for its life). I'm thankful that I'm not ethically permitted to decapitate a rat, because it's terrifying watching a living creature being killed like that, let alone doing it. My only job in this process is to wipe up the arterial blood spurt and wash the bloody equipment. Until now I can still remember the rat's eyes as they widen with fear when the blades hit all 4 sides of its neck, and that gaping mouth to let out one last unheard squeak. I don't think I will forget this, ever.
Ok, I think this will do for now. Time for dinner!
Thursday, July 23, 2009
Lunchbreak!
Nom nom I love corn...
Had an enlightening conversation over lunch about the age that people want to get married.
Getting tired of literature review, as I find it impossible to absorb anything on screen, but feel guilty printing all the time.
Brain stops...
...here.
Wednesday, July 22, 2009
New blog appearance
Some photos of the past few days...
Sunday, July 19, 2009
Another weekend on the other side of the world...
Friday, July 17, 2009
Thursday, July 16, 2009
A weekend of exploration
Photos will unfortunately not yet be forthcoming, as my computer is in at the Apple store (so nice to live within walking distance of one) for repairs. I am hoping to have it with me again my Wednesday, but we shall see.
We explored not only the colleges, but also the many laneways and sidestreets of Cambridge, and walked up and down the banks of the Cam. Punters were out in force, and it was amusing to note the difference between the graceful, effortless control exhibited by the professionals, and the directionless meandering of the first time punters. Particularly chuckle-worthy was an incident in which someone had planted the pole and been unable to twist it free, and had therefore been forced to watch as their pole remained fixed in the river bottom while their punt sailed blindly forward. I unfortunately didn't have the chance to see (and photograph for posterity's sake) an upset punt or a punter left in the river after trying too hard to free their pole. This had apparently happened not long before we arrived on the Backs of John's, as there were a number of males dressed in nothing but underpants and looking rather wet. It appeared that one had fallen in and the rest had followed, deciding to swim if that were the present fashion.
We ate at a number of interesting places. The Lettuce and Slug was an interesting modern pub, serving some kind of Mexican/Italian fusion food, while The Mitre was very much a traditional pub, complete with drunkards. A group of young men, and one old man, were enjoying rather too much of the local ale, and much hilarity ensued upon their attempts to walk, talk, or drink from a glass. Also in that pub, Hon struck up a conversation with our neighbouring table, and perhaps only in Cambridge would you discover that a table of four similarly-aged, multinational people are conducting research into carbon nanotubes and molecular genetics. Their nationalities would have suggested a Contiki tour; two Turks, a Pole, and a Frenchman.
Food here, at least in restaurants, is proving a deal more exciting than expected. It seems that even staid old England is coming to realise that not everyone eats meat, and is thus beginning to provide vegetarian options. Even food in the supermarket is labelled "vegetarian" when it satisfies the criteria. Even cheeses note whether they are suitable for vegetarians or not (whether their rennent is made from processed calf). I had a delicious baked potato with garlic mushrooms at "Tatties", a potato specialist restaurant.
I'll post this now and write more later. I'm getting frustrated with how long it's taking the bank to send me my pin, as well as the fact that my computer is not back yet!
Convergence
- ethics not done, supervisor n00b at it, due monday, supervisor leaving for overseas on Sunday for a week
- someone pulling out of seeing Harry Potter with me and refusing to reimburse me or to find someone to take the ticket
- problems with new property
Tuesday, July 14, 2009
stocks are low?
Awesome.
Monday, July 13, 2009
a tale of two burnings
It was the best of foods, it was the worst of foods, it was the age of wisdom, it was the age of pressing too many buttons, it was the epoch of belief, it was the epoch of disbelief as I walked back into the kitchen, it was the season of Light (fire), it was the season of Darkness (rice+bowl), it was the spring of cluelessness, it was the winter of total fail, we had everything before us, we had nothing before us but smoke inhalation, we were all going direct to AMS tomorrow, we were all going direct to lung cancer - in short, the period was so far like when I was eight and mum said "10 minutes" in the microwave and not "10 seconds".
---
It was the year two thousand and nine. Culinary and academic revelations were conceded to me at that favoured period, as at this. I had recently attended my first blessed day at AMS, bestowed with a paltry three hours sleep. Even the prospect of a full day ahead did not remove the thought of the smoky microwave from my mind. At 12:30 am that morning, I had risen from bed and attempted to clean it. Even at 2:30 in the morning it stressed me. I believe I got up at 6 am.
The Royal Children's Hospital, less favoured on the whole as to matters holiday-related than her sister of the late bedtime and lazy breakfast, loomed with exceeding ugliness up the hill, making my day several hours too long. Under the guidance of Michael Marks, she gave us an insanely boring two-hour seminar on how to use Ovid to search for things, as well as a rambling talk on Clinical Research something-something (quality? This was at about the time when the lack of sleep kicked in). It is likely enough that, rooted in the yonks of experience of the RCH AMS unit, they have good reason for doing this. It is likely enough that my grudging acceptance of this may have been slightly (very) influenced by the excellent morning tea of berry friands, muffins and jam donuts. And tea. Lovely tea.
In the Fourth Floor of the RCH, there was scarcely an amount of order and excitement to justify much blogging. Daring powerpoints by men munching covertly on potato chips, and seminar-way robberies of my valuable brainspace and time, will take place every two weeks, compulsorily; victims... I mean, students, were publicly cautioned not to go out of town at these meeting times; the students will annoy the supervisors, and the supervisors will annoy the students, and nobody thought any of these occurrences much out of the common way. In the midst of them, the hangman of boredom, ever busy and ever worse than useless, was in constant requisition; now, stringing up long rows of miscellaneous criminals; now, hanging a lazy kid on Saturday who had been told off on Tuesday; now, burning people in the hand with whatever came out of the microwave, and now burning rice at the door of an apartment on Lygon Street; today, taking the life of a rather stupid girl, and tomorrow of my dad who is gonna come over for a little while (he says that smoke smells take forever to come out).
All these things, and a thousand like them, will come to pass in and close upon the dear old year two thousand and nine. Environed by them, while I work unheeded, trod with total randomness, and carried their rights in a plastic bag that gets shoved under the seat when I need to be Asian. Thus did the year two thousand and nine conduct their Lessnesses, and myriads of small creatures - the creatures of this chronicle among the rest - along the roads that lay before them.
Friday, July 10, 2009
Administration thing
Thursday, July 9, 2009
It truly begins...
Tomorrow will be another 8-6(:30) day, I think, but at least I will get to relax somewhat on the weekend. Hon is coming to visit on his way to the US of A, and therefore I will have the opportunity to do some more exploration of Cambridge, along with buying a much-needed bike, taking photos, and (fingers crossed) getting my phone/internet sorted out. As always, the weather is highly unpredictable, but it has cooled down a lot; something for which I am very grateful. I do notice how little UV there is here, though. Even at midday on a bright sunny day, my glasses take a good half hour before they are even slightly dark, and never get very dark no matter how long I am in the sun (for reference; on an overcast Melbourne morning they are fairly dark within 10 minutes). No wonder English people end up red and blistered after half a day in Australia! I wonder how anyone here gets vitamin D...
Of Worms, Dogs and Men
I will assign the people mentioned in this entry (and henceforth any entry after this)aliases.
Of Worms
Hong: so are we the only Australian group in Coeliac Disease research?
#2: well, there's the worm people up in Queensland that you work with, no?
$: yes. This gastroenterologist infected himself with hookworm when he went to PNG. He just put one on his arm and let it burrow through...
Hong: ...
$: he had them for years. And he purified the his faeces and infected other people. So he's got a research group on worms going now.
Hong: ... WHAT?
#2: and the Americans really couldn't cope with it! When we told them at the conference, they were like,"oh my god!!!" They were so horrified they really couldn't cope with the idea. They were asking,"how on earth did he get ethics?"
Hong: and how on earth DID he get ethics?
$: well, all it takes is someone you know on the committee. Easy.
Of Dogs
Russian: once, after one particular long day at the lab, I got home. And instead of my dog jumping up and down and wagging his tail like he normally does, he was literally crawling to me! At once I freaked out and picked him up. Was he sick? Maybe there was some spine problem? I was just about to ring the vet when I walked into the kitchen and saw this massive amount of poo on the floor. I was so relieved! If that's the only thing wrong then it's easily cured!
Everyone: XD
Russian: then I remembered my high school friend in Russia. He went home one day and said to his parents,'I've got a brain tumour.' And his parents really freaked out. Then he said,'no, actually I don't have a brain tumour. I just wanted to tell you I failed my exams.'
Everyone: ...
Of Men
$: Made a speedy recovery, did you?
#1 (recently underwent major surgery; mind you, he's a consultant himself): Well, I literally conned my way out of the hospital. I said to the nurses on Shift 1,'They told me I'm going home tomorrow.' They said,'ah really, that's great!' then I said to nurses on Shift 2,'I'm going home tomorrow!' They said,'congrats, you're doing well!' then the nurses on Shift 3 said to me,'are you going home today?' and I said,'yes, that's what they've told me.' Then my surgeon came,'so, your discharge is scheduled on Friday.' I said,'no it isn't. They told me I'll be discharged today!' and he said,'oh, really? okay.' I conned my way out by creating a rumour!
Everyone: XP
#2: And how was it at home?
#1: It was great! I did my own heparin injections for 5 days. You know, jabbing at the tummy. My wife wasn't very impressed. 'What're you doing?' she asked. 'Just shooting up some drugs.' I said.
$: Ha. They did give you some real stuff though, didn't they?
#1: Yes. Oxycortin. Nasty stuff. I hate narcotics. I've started doing some email reviews for the past month... er... *grimace* very blunt, not good...
$: XD. Wow, so you were showing your true self, then.
#1: Well, I wouldn't say that. Because normally I'm on alcohol anyway.
Tuesday, July 7, 2009
Thunderstorm
Bumblebee
AMS Begins
Monday, July 6, 2009
AMS: Day 0, anyone?
Though there's almost nothing new in the level 5 lab of Dept Immuno & Micro, it's still somewhat exciting - and that's not because I'm not getting paid like I was during summer holidays.
I thought day 1 would be full of red-tape. But turned out my supervisor wasn't organised enough for that - swine flu had struck her the previous week so she had been quite behind and stressed out. So in the end the only red-tape I did was to renew my access card. So, a day 0 instead.
Day 0 turned out to be "educational". In addition to about 20 more papers to read, I strolled across Royal Parade to WEHI, catching sight of Geoff McColl jaywalking towards RMH on my way XD, and fell asleep half way through the Autoimmunity Postdoc seminar so lost track of what the Professor's new findings were... *sigh*
The only useful thing was having a talk with the supervisor regarding the project. What I had found out, much to my surprise, was that I would be required to be absolutely write down everything, and date and get my supervisor to sign every single page of the book... Unfortunately my project will be part of the contracted work our lab does to an external company... hence the extreme pedanticness. If only I am OCD enough...
The rest of the talk was all within my expectations, since I have been in the lab before. Things like "you know you will proabably be working more than 9-5, right?"; "when we have experiments going, you'll be expected to come in on weekends..."; "our internet should not be used for purposes other than work-related... of course, that is not to say you can't game or whatever at all, if you're the only person in the lab at 8 or 9 at night and running a very long experiment." Ah well, such is lab, and I have been expecting the worst anyway since learning that the phD students often come in at 6am and leave at 9pm...
So that's about all for my 1st day. Somewhat sluggish, but then again, the momentum will probably only pick up 1 month into AMS, if I'm lucky.
And now I want to hear everyone else's AMS Day 1 (or Day 0, if you've got a worse than red-tape day like me)... Post away!
Sunday, July 5, 2009
A Man from Mukinupin
Despite being heralded as a great Australian theatrical creation, the play felt rather like a conjured up pieces of information - of course, all of them relating to this little country town, but seemed rather random between themselves. This resulted in me wondering where the story is leading to at the half-time. During the 2nd half, the play did tie its loose ends together, however it seems rather contrived, and the result was both puzzling and comical. Perhaps, had the play trimmed itself a little to allow more character and plot development, had it actually been a book where people can read and absorb rather than feeling being "rushed" to the conclusion, would have made this a truly deep, meaningful and great work. I wonder how much of its failure to captivate the audience can be attributed to the playwright, and how much to the director and his/her interpretation and presentation.
The bright side of the play, though, was the excellent sets. White sand, a real fire, a "magic" caravan that all characters can mysteriously appear and disappear from, and a rainy scene, all on stage, are but a few. Not to mention the 2 main female and male casts were playing 2 different characters each - which, one wonders at the speed of their clothes change, behind the magic caravan. The use of curtains as a prop was a clever design, for it allowed artistic use of the shadows and created the right atmosphere for the many of the settings in the play.
Overall, an experimental piece of work... but it would seem to me that the effort in making the technological marvel on stage has perhaps compromised the depth of the story... and for any play, no flashy settings can replace a strong plot and good character development...